pVHed02
(Plasmid #82352)

• Purpose: Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid
• Depositing Lab: Richard Murray
• Publication: Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663.

Backbone

• Vector backbone: pSC101
• Backbone size w/o insert (bp): 3400
• Total vector size (bp): 5676
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mKate2 RFP
• Insert Size (bp): 696

Gene/Insert 2

• Gene/Insert name: Superfolder GFP
• Insert Size (bp): 720

Gene/Insert 3

• Gene/Insert name: Temporal logic gate with TP901 and Bxb1 attachment sites
• Insert Size (bp): 394

Resource Information

• Supplemental Documents:
  • pVHed02 annotated plasmid map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry