pcDNA 3.1 (+) Tacδ
(Plasmid
#81177)
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PurposeMammalian expression of human Tacδ (GFFKR cytoplasmic tail)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 81177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)hygro
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTacδ
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Alt nameCD25
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SpeciesH. sapiens (human)
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MutationCD25 amino acids 1 to 263 fused to KLGFFKR cytoplasmic tail
- Promoter CMV
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Tag
/ Fusion Protein
- KLGFFKR (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1 (+) Tacδ was a gift from Chinten James Lim (Addgene plasmid # 81177 ; http://n2t.net/addgene:81177 ; RRID:Addgene_81177) -
For your References section:
The membrane-proximal KXGFFKR motif of alpha-integrin mediates chemoresistance. Liu CC, Leclair P, Yap SQ, Lim CJ. Mol Cell Biol. 2013 Nov;33(21):4334-45. doi: 10.1128/MCB.00580-13. Epub 2013 Sep 3. 10.1128/MCB.00580-13 PubMed 24001772
