Pmyo-3::tag:SL1(G20A)
(Plasmid #81114)

• Purpose: Expression of exogenous SL1(G20A) in C. elegans muscle
• Depositing Lab: Xiao Liu
• Publication: Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw734.

Backbone

• Vector backbone: pEASY-Blunt Simple Cloning Vector
• Backbone manufacturer: Transgen
• Backbone size w/o insert (bp): 3830
• Total vector size (bp): 6364
• Vector type: Worm Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sls-1.2
• Alt name: SL1
• Species: C. elegans (nematode)
• Insert Size (bp): 2354
• Mutation: G20A
• Entrez Gene: sls-1.2 (a.k.a. CELE_Y102A5D.14)
• Promoter: promoter of myo-3
• Tag / Fusion Protein:
  • illumina-adapter (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Joel H. Rothman's lab (Mol Cell Biol. 1999 Mar;19(3):1892-900)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry