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Addgene

pUB_smFLAG_ActB_MS2
(Plasmid #81083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 81083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-UbiC
  • Total vector size (bp) 10118
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    smFLAG_ActB_24xMS2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUB_smFLAG_ActB_MS2 was a gift from Tim Stasevich (Addgene plasmid # 81083 ; http://n2t.net/addgene:81083 ; RRID:Addgene_81083)

  • For your References section:

    Real-time quantification of single RNA translation dynamics in living cells. Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ. Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899. Epub 2016 May 5. 10.1126/science.aaf0899 PubMed 27313040
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