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Purposemammalian expression of Smad4
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80888 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmad4
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Alt nameSMAD family member 4
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SpeciesH. sapiens (human)
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Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Smad4 insert contains a D2E mutation. The depositor states that this mutation does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Flag-Smad4 was a gift from Aristidis Moustakas (Addgene plasmid # 80888 ; http://n2t.net/addgene:80888 ; RRID:Addgene_80888) -
For your References section:
Functional consequences of tumorigenic missense mutations in the amino-terminal domain of Smad4. Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH. Oncogene. 2000 Sep 7;19(38):4396-404. 10.1038/sj.onc.1203798 PubMed 10980615
