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Addgene

pcDNA3-ALK7 K222R
(Plasmid #80887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALK7
  • Alt name
    ACVR1C
  • Alt name
    activin A receptor type 1C
  • Species
    R. norvegicus (rat)
  • Mutation
    K222R
  • Entrez Gene
    Acvr1c (a.k.a. Alk-7, Alk7, habrec1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ALK7 K222R was a gift from Aristidis Moustakas (Addgene plasmid # 80887 ; http://n2t.net/addgene:80887 ; RRID:Addgene_80887)

  • For your References section:

    Functional consequences of tumorigenic missense mutations in the amino-terminal domain of Smad4. Moren A, Itoh S, Moustakas A, Dijke P, Heldin CH. Oncogene. 2000 Sep 7;19(38):4396-404. 10.1038/sj.onc.1203798 PubMed 10980615
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