pTSA29
(Plasmid
#80778)
-
Purpose(Empty Backbone) Cloning vector with temperature sensitive origin of replication.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepWSK29
-
Backbone manufacturerWang, R.F. and S. R. Kushner. 1991. Gene 100:195-199.
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13/pUC Forward
- 3′ sequencing primer M13/pUC Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTSA29 was a gift from Gregory Phillips (Addgene plasmid # 80778 ; http://n2t.net/addgene:80778 ; RRID:Addgene_80778) -
For your References section:
New cloning vectors with temperature-sensitive replication. Phillips GJ. Plasmid. 1999 Jan;41(1):78-81. 10.1006/plas.1998.1380 PubMed 9887310
Map uploaded by the depositor.
