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Addgene

pTSK29
(Plasmid #80779)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80779 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWSK29
  • Backbone manufacturer
    Wang, R.F. and S. R. Kushner. 1991. Gene 100:195-199.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13/pUC Forward
  • 3′ sequencing primer M13/pUC Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTSK29 was a gift from Gregory Phillips (Addgene plasmid # 80779 ; http://n2t.net/addgene:80779 ; RRID:Addgene_80779)
  • For your References section:

    New cloning vectors with temperature-sensitive replication. Phillips GJ. Plasmid. 1999 Jan;41(1):78-81. 10.1006/plas.1998.1380 PubMed 9887310