Pvha6-AgDD
(Plasmid #80626)

• Purpose: create protein aggregates in C. elegans
• Depositing Lab: Thomas Wandless
• Publication: Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.

Backbone

• Vector backbone: modified worm expression vector with Pvha6 promoter
• Backbone size w/o insert (bp): 4607
• Total vector size (bp): 5697
• Modifications to backbone: same insert as Plasmid #78289 (AgDD), but with with Pvha6 promoter for worm expression.
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NES-L106P-sfGFP
• Alt name: AgDD
• Species: H. sapiens (human)
• Insert Size (bp): 1087
• GenBank ID: NM_000801 AB971579
• Entrez Gene: FKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
• Tag / Fusion Protein:
  • MLALKLAGLDI (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor-provided reference sequence shows 3 additional nucleotide mismatches compared to listed NM_000801 and also F36V compared to the respective protein (NP_000792.1). Addgene Sanger sequencing confirmed these differences.