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Addgene

pJOG252
(Plasmid #80584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVM_BGW-BpiI
  • Backbone manufacturer
    6800
  • Total vector size (bp) 16000
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    pnos:nptII-tnos
  • Alt name
    Kanamycin resistance, plant selectable
  • Species
    E. coli
  • Insert Size (bp)
    1800

Gene/Insert 2

  • Gene/Insert name
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
  • Alt name
    dCas9-AD(TAL) fusion
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    5800
  • Mutation
    D10A

Gene/Insert 3

  • Gene/Insert name
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
  • Alt name
    sgRNA+dummy
  • Insert Size (bp)
    1700

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJOG252 was a gift from Johannes Stuttmann (Addgene plasmid # 80584 ; http://n2t.net/addgene:80584 ; RRID:Addgene_80584)

  • For your References section:

    Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. 10.1111/tpj.13319 PubMed 27579989
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