pJOG251
(Plasmid #80583)

• Purpose: recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
• Depositing Lab: Johannes Stuttmann
• Publication: Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.

Backbone

• Vector backbone: pVM_BGW-BpiI
• Backbone manufacturer: 6800
• Total vector size (bp): 15900
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pnos:PAT-tnos
• Alt name: BASTA resistance, plant selectable
• Species: E. coli
• Insert Size (bp): 1800

Gene/Insert 2

• Gene/Insert name: p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
• Alt name: dCas9-AD(TAL) fusion
• Species: Streptococcus pyogenes
• Insert Size (bp): 5800
• Mutation: D10A

Gene/Insert 3

• Gene/Insert name: BsaI-ccdB_CmR-BsaI
• Alt name: dummy
• Insert Size (bp): 1500

Resource Information

• Supplemental Documents:
  • pJOG251_annotated_seq

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511