pDGE78
(Plasmid #80580)

• Purpose: recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII
• Depositing Lab: Johannes Stuttmann
• Publication: Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.

Backbone

• Vector backbone: pVM_BGW
• Backbone manufacturer: 6800
• Total vector size (bp): 15400
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pnos:nptII-tmos
• Alt name: Kanamycin resistance, plant selectable
• Species: E. coli
• Insert Size (bp): 1800

Gene/Insert 2

• Gene/Insert name: pPcUbi:Cas9 D10A-tnos
• Alt name: Cas9 nickase
• Species: Streptococcus pyogenes
• Insert Size (bp): 5300
• Mutation: D10A

Gene/Insert 3

• Gene/Insert name: BsaI-ccdB_CmR-BsaI
• Alt name: dummy
• Insert Size (bp): 1500

Resource Information

• Supplemental Documents:
  • pDGE078_annotated_seq

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry