pDGE77
(Plasmid
#80579)
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Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVM_BGW
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Backbone manufacturer6800
- Total vector size (bp) 15400
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namepnos:PAT-tnos
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Alt nameBASTA resistance, plant selectable
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SpeciesE. coli
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Insert Size (bp)1800
Gene/Insert 2
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Gene/Insert namep2x35S:Cas9 D10A-tnos
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Alt nameCas9 nickase
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SpeciesStreptococcus pyogenes
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Insert Size (bp)5300
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MutationD10A
Gene/Insert 3
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Gene/Insert nameBsaI-ccdB_CmR-BsaI
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Alt namedummy
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Insert Size (bp)1500
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDGE77 was a gift from Johannes Stuttmann (Addgene plasmid # 80579 ; http://n2t.net/addgene:80579 ; RRID:Addgene_80579) -
For your References section:
Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. 10.1111/tpj.13319 PubMed 27579989