pPtPBR11
(Plasmid
#80386)
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Purpose(Empty Backbone) Episome vector for Phaeodactylum tricornutum (pBR322-based)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 80386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size (bp) 4360
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Modifications to backboneAddition of yeast CEN6-ARSH4 and partial HIS3, Phaeodactylum tricornutum ShBle cassette, origin of transfer from RP4
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Vector typeSynthetic Biology ; : Episomal vector for Phaeodactylum tricornutum
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Selectable markersZeocin, HIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequence is associated with the manuscript Diner et al. 2016 in review
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPtPBR11 was a gift from Philip Weyman (Addgene plasmid # 80386 ; http://n2t.net/addgene:80386 ; RRID:Addgene_80386)