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Addgene

pPtPBR11
(Plasmid #80386)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80386 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Backbone size (bp) 4360
  • Modifications to backbone
    Addition of yeast CEN6-ARSH4 and partial HIS3, Phaeodactylum tricornutum ShBle cassette, origin of transfer from RP4
  • Vector type
    Synthetic Biology ; : Episomal vector for Phaeodactylum tricornutum
  • Selectable markers
    Zeocin, HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence is associated with the manuscript Diner et al. 2016 in review

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPtPBR11 was a gift from Philip Weyman (Addgene plasmid # 80386 ; http://n2t.net/addgene:80386 ; RRID:Addgene_80386)