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pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)
(Plasmid #80004)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 80004 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC4EN-F1
  • Backbone manufacturer
    ARIAD Pharmaceuticals, Inc.
  • Backbone size w/o insert (bp) 1312
  • Total vector size (bp) 2978
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FKBP
  • Insert Size (bp)
    966
  • Promoter CMV
  • Tags / Fusion Proteins
    • GAF domain of miRFP709 (C terminal on insert)
    • NLS (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709) was a gift from Vladislav Verkhusha (Addgene plasmid # 80004 ; http://n2t.net/addgene:80004 ; RRID:Addgene_80004)

  • For your References section:

    Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV. Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. 10.1038/ncomms12405 PubMed 27539380
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