pBGC155-MXMT
(Plasmid #79818)

• Purpose: GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)
• Depositing Labs: Yutaka Kodama, Masamitsu Wada
• Publication: Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 10.1007/s11103-009-9467-0. Epub 2009 Feb 14.

Backbone

• Vector backbone: CaMV35S- sGFP(S65T)-NOS vector
• Backbone manufacturer: Chiu W
• Backbone size w/o insert (bp): 4180
• Modifications to backbone: cDNA fragments of CaMXMT1 (Uefuji et al. 2003) were subcloned into the BsrGI/NotI sites of pBGC155.
• Vector type: Plant Expression ; Reporter plasmid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CaMXMT1
• Alt name: MXMT
• Species: Synthetic
• Insert Size (bp): 1137
• Promoter: CaMV 35S promoter
• Tag / Fusion Protein:
  • GC155/GFP (155–239) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGⅠ (not destroyed)
• 3′ cloning site: NotⅠ (not destroyed)
• 5′ sequencing primer: TATATGTACAAGATGGAGCTCCAAGAAGTC
• 3′ sequencing primer: TATAGCGGCCGCTTACACGTCTGACTTCTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry