RP4-11
(Plasmid #79812)

• Purpose: Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid
• Depositing Lab: Michael Hynes
• Publication: Quandt et al Plasmid. 2004 Jul;52(1):1-12.

Backbone

• Vector backbone: RP4
• Backbone manufacturer: natural plasmid
• Backbone size w/o insert (bp): 60000
• Total vector size (bp): 60004
• Modifications to backbone: Insertion of KmSacB cassette by in vivo recombination. For details see original Quandt et al. paper from 2004.
• Vector type: Helper plasmid for mobilization

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): CSH56
• Growth instructions: Select for the chromosome with nalidixic acid.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Kanamycin cassette and SacB gene
• Alt name: Km, SacB
• Insert Size (bp): 2800
• Entrez Gene: sacB (a.k.a. BSU_34450, BSU34450)
• Promoter: Km

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We made this plasmid by in vivo recombination

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is designed to be used in mobilizing other plasmids, and is easily curable from recipient cells because of the sacB gene. Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity.

Depositor states that users can select for the chromosome with nalidixic acid.