GST-SF3B2 R508K aa 401-550 fragment
(Plasmid #79674)

• Purpose: bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation
• Depositing Lab: Steven Clarke
• Publication: Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.659433.

Backbone

• Vector backbone: pGEX-6p-1
• Backbone manufacturer: GE healthcare
• Backbone size w/o insert (bp): 4984
• Total vector size (bp): 5435
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: splicing factor 3b subunit 2
• Alt name: SF3B2
• Alt name: SAP145
• Species: H. sapiens (human)
• Insert Size (bp): 451
• Mutation: aa 451-550 with R508K
• GenBank ID: NM_006842.2
• Entrez Gene: SF3B2 (a.k.a. CFM, Cus1, HFM, SAP145, SF3B145, SF3b1, SF3b150)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mark Bedford, The University of Texas MD Anderson Cancer Center, Smithville, Texas

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry