pMpGWB102-Citrine-NLS
(Plasmid #79136)

• Purpose: Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants
• Depositing Lab: Yutaka Kodama
• Publication: Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.

Backbone

• Vector backbone: pMpGWB102
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10404
• Total vector size (bp): 11145
• Modifications to backbone: These PCR-amplified DNA fragments of Citrine-NLS were cloned into a pDONR207 plasmid by BP reaction (Invitrogen). The resulting plasmids were mixed with pMpGWB102 vector, and LR reaction (Invitrogen) was performed to generate pMpGWB102-Citrine-NLS.
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Citrine-NLS
• Species: Synthetic
• Insert Size (bp): 744
• Promoter: CaMV 35S promoter
• Tag / Fusion Protein:
  • NLS (Nuclear Localization Signal) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGTGAGCAAGGGCGAG
• 3′ sequencing primer: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACACCTTGCGCTTCTTCTTAGGAGGCTTGTACAGCTCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry