pMpGWB106-MTS-Citrine
(Plasmid
#79135)
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PurposeBinary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 79135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMpGWB106
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 11124
- Total vector size (bp) 11907
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSD3(1-50)
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Alt nameMTS
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)150
- Promoter CaMV 35S promoter
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Tag
/ Fusion Protein
- Citrine (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGATGATGATGAATCT
- 3′ sequencing primer GGGGACCACTTTGTACAAGAAAGCTGGGTCCTTTGAAAGAAATGAGTT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMpGWB106-MTS-Citrine was a gift from Yutaka Kodama (Addgene plasmid # 79135 ; http://n2t.net/addgene:79135 ; RRID:Addgene_79135) -
For your References section:
Cold-induced organelle relocation in the liverwort Marchantia polymorpha L. Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y. Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085. 10.1111/pce.12085 PubMed 23421791