Cas9-GFP_sg_mAMPKa1
(Plasmid #79004)

• Purpose: Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.
• Depositing Lab: Reuben Shaw
• Publication: Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.

Backbone

• Vector backbone: pSpCas9(BB)-2A-GFP (PX458)
• Total vector size (bp): 9300
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Prkaa1
• gRNA/shRNA sequence: TTATTGTCACAGGCATATGG
• Species: M. musculus (mouse)
• Entrez Gene: Prkaa1 (a.k.a. AMPKalpha1, C130083N04Rik)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: GAGGGCCTATTTCCCATGATTCC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry