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PCS4 3XFLAG-PPARgamma1
(Plasmid #78769)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS4-3FLAG
  • Backbone manufacturer
    derived from pCS2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR_1
  • Alt name
    isoform 1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCS4+ is based on pCS2+, with the following unique restriction endonuclease sites are added to MCS: BsrG1, EcoRV, AscI, NcoI, EcoO019, Sfi I, Fes I, Bgl II, Nhe I. A start codon with the Kozak sequence and stop codons in all three frames are added.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCS4 3XFLAG-PPARgamma1 was a gift from Jaewhan Song (Addgene plasmid # 78769 ; http://n2t.net/addgene:78769 ; RRID:Addgene_78769)

  • For your References section:

    Suppression of PPARgamma through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation. Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J. Cell Death Differ. 2014 Apr;21(4):594-603. doi: 10.1038/cdd.2013.181. Epub 2013 Dec 13. 10.1038/cdd.2013.181 PubMed 24336050
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