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Purpose(Empty Backbone) Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIJ8668
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Modifications to backboneSee publication for cloning details
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Vector typeBacterial Expression ; Streptomyces expression
- Promoter inducible PnitA-NitR system
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5'-gcatgctaaaccgatacaatt-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank reference sequence JN636796 for NitR
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL99 was a gift from Xuming Mao (Addgene plasmid # 78577 ; http://n2t.net/addgene:78577 ; RRID:Addgene_78577) -
For your References section:
Construction of over-expression shuttle vectors in Streptomyces. Sun N, Wang Z-B, Wu H-P, Mao X-M and Li Y-Q. Annals of Microbiology Dec 2012, Volume 62, Issue 4, pp 1541-1546 10.1007/s13213-011-0408-1
Map uploaded by the depositor.
