EcMscS
(Plasmid
#78555)
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PurposeE. coli Mechanosensitive Channel of Small Conductance
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 78555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5364
- Total vector size (bp) 6222
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMechanosensitive Channel of Small Conductance
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Alt nameMscS
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SpeciesE. coli
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Insert Size (bp)858
- Promoter T7 Promoter
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Tag
/ Fusion Protein
- 6x Histidine Tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EcMscS was a gift from Douglas Rees (Addgene plasmid # 78555 ; http://n2t.net/addgene:78555 ; RRID:Addgene_78555) -
For your References section:
Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Bass RB, Strop P, Barclay M, Rees DC. Science. 2002 Nov 22;298(5598):1582-7. 10.1126/science.1077945 PubMed 12446901
