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PurposeExpresses Murine Leukemia Reverse Transcriptase (MLRT)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78234 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVRb
- Backbone size w/o insert (bp) 3514
- Total vector size (bp) 5742
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMurine leukemia reverse transcriptase
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SpeciesSynthetic
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Insert Size (bp)2001
- Promoter J23102
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer na
- 3′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMLRT was a gift from Christopher Voigt (Addgene plasmid # 78234 ; http://n2t.net/addgene:78234 ; RRID:Addgene_78234) -
For your References section:
Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Elbaz J, Yin P, Voigt CA. Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179. 10.1038/ncomms11179 PubMed 27091073