pHIV_pTp66p51
(Plasmid #78233)

• Purpose: Expresses HIV reverse transcriptase
• Depositing Lab: Christopher Voigt
• Publication: Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10.1038/ncomms11179.

Backbone

• Vector backbone: pSB1A3
• Total vector size (bp): 6622
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: p66
• Species: Synthetic
• Insert Size (bp): 1686
• Promoter: pTac Minimal Promoter

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: p51
• Species: Synthetic
• Insert Size (bp): 1326
• Promoter: pTac Minimal Promoter

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Gene/Insert 3

• Gene/Insert name: LacI
• Species: Synthetic
• Insert Size (bp): 1383
• Promoter: Const. Promoter (From DNA2.0 Vec)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry