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Addgene

pGEX6p-Meis1a
(Plasmid #78144)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 78144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone manufacturer
    GE Healthcare
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Meis1a
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Meis1 (a.k.a. C530044H18Rik, Evi8)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6p-Meis1a was a gift from Francesco Blasi (Addgene plasmid # 78144 ; http://n2t.net/addgene:78144 ; RRID:Addgene_78144)

  • For your References section:

    Prep1 and Meis1 competition for Pbx1 binding regulates protein stability and tumorigenesis. Dardaei L, Longobardi E, Blasi F. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):E896-905. doi: 10.1073/pnas.1321200111. Epub 2014 Feb 27. 10.1073/pnas.1321200111 PubMed 24578510
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