pINDUCER21-PGBD5
(Plasmid
#78121)
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PurposeLentiviral gene expression vector for doxycycline inducible PGBD5 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 78121 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepINDUCER21
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Backbone manufacturerStephen Elledge, Thomas Westbrook
- Backbone size w/o insert (bp) 12152
- Total vector size (bp) 13878
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePGBD5
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SpeciesH. sapiens (human)
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Insert Size (bp)3181
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GenBank IDNM_001258311.2
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Entrez GenePGBD5
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note the NCBI reference (NP_001245240.1/NM_001258311.2) for this sequence may differ from the publication. Please view the sequence prior to requesting this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pINDUCER21-PGBD5 was a gift from Alex Kentsis (Addgene plasmid # 78121 ; http://n2t.net/addgene:78121 ; RRID:Addgene_78121) -
For your References section:
PGBD5 promotes site-specific oncogenic mutations in human tumors. Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still E, MacArthur IC, Rodriguez-Fos E, Gonzalez S, Puiggros M, Blackford AN, Mason CE, de Stanchina E, Gonen M, Emde AK, Shah M, Arora K, Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CWM, Steen H, Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A. Nat Genet. 2017 May 15. doi: 10.1038/ng.3866. 10.1038/ng.3866 PubMed 28504702