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Addgene

pTrcECT
(Plasmid #75477)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75477 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrc99a
  • Backbone manufacturer
    Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ectABC
  • Species
    E.coli
  • Insert Size (bp)
    2550
  • GenBank ID
    9747780, 9747563 and 9747564
  • Promoter trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer PTrcPro-F acaattaatcatccggctcg
  • 3′ sequencing primer rrnB-T1-term-Rev GAAAGGCCCAGTCTTTCGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrcECT was a gift from Xixian Xie (Addgene plasmid # 75477 ; http://n2t.net/addgene:75477 ; RRID:Addgene_75477)

  • For your References section:

    Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli. Ning Y, Wu X, Zhang C, Xu Q, Chen N, Xie X. Metab Eng. 2016 Mar 9;36:10-18. doi: 10.1016/j.ymben.2016.02.013. 10.1016/j.ymben.2016.02.013 PubMed 26969253
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