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pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2
(Plasmid #75295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 75295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene Plasmid #50465
  • Backbone manufacturer
    Bryan Roth
  • Backbone size w/o insert (bp) 4554
  • Total vector size (bp) 6854
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eGFP-2A-mKv1.2
  • Alt name
    eGFP
  • Alt name
    murine potassium channel
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2300
  • Entrez Gene
    Kcna2 (a.k.a. Akr6a4, Gm10672, Kca1-2, Kv1.2, Mk-2)
  • Promoter human SYN1

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer hSYN1 F417 actcagcgctgcctcagtct
  • 3′ sequencing primer WPRE_R1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2 was a gift from Brandon Harvey (Addgene plasmid # 75295 ; http://n2t.net/addgene:75295 ; RRID:Addgene_75295)

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