p3E-MCS
(Plasmid #75174)

• Purpose: Multiple cloning site for insertion of gene of interest for C-terminal fusions.
• Depositing Lab: Nicholas Cole
• Publication: Don et al Zebrafish. 2016 Sep 15.

Backbone

• Vector backbone: pDONRP4P1R
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2646
• Total vector size (bp): 2709
• Vector type: Three prime entry vector for Multisite Gateway Three Fragment vector construction kit (Invitrogen)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Multiple Cloning Site
• Insert Size (bp): 63
• Promoter: n/a

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The multiple cloning site insert was purchased from GeneArt™ Genestring™ Fragment (Invitrogen/ ThermoFisher Scientific)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry