pCX-2A-2A
(Plasmid
#74670)
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Purpose(Empty Backbone) based on pCX-C1 with F2A1/F2A2 inserted
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74670 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCX-C1 (a modified version of pCX-EGFP plasmid from Okabe M., et al., FEBS Letters, 407 (1997) 313–319)
- Backbone size (bp) 5990
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Modifications to backboneinsertion of 2A-2A (F2A1/F2A2)
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Vector typeMammalian Expression
- Promoter pCAGGS
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional details can be found in the following publication:
Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-alpha-Induced Injury In Vitro. PLoS One. 2015 Oct 29;10(10):e0141933. doi: 10.1371/journal.pone.0141933. eCollection 2015.
PMID: 26513260
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCX-2A-2A was a gift from Roberto Giovannoni (Addgene plasmid # 74670 ; http://n2t.net/addgene:74670 ; RRID:Addgene_74670) -
For your References section:
Co-expression of functional human Heme Oxygenase 1, Ecto-5'-Nucleotidase and ecto-nucleoside triphosphate diphosphohydrolase-1 by "self-cleaving" 2A peptide system. De Giorgi M, Cinti A, Pelikant-Malecka I, Chisci E, Lavitrano M, Giovannoni R, Smolenski RT. Plasmid. 2015 May;79:22-9. doi: 10.1016/j.plasmid.2015.03.004. Epub 2015 Mar 14. 10.1016/j.plasmid.2015.03.004 PubMed 25779031
