NUDT1
(Plasmid
#74660)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETM-11
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNUDT1
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Alt nameNUDT1A-c001
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Alt nameNUDT1Anudix-type motif 1 isoform p18 [tv1]
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SpeciesH. sapiens (human)
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Insert Size (bp)549
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Entrez GeneNUDT1 (a.k.a. MTH1)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
T7/lac regulated, N-terminal His-tag, TEV, restriction cloned with NcoI/KpnI gi|40288274
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NUDT1 was a gift from Nicola Burgess-Brown (Addgene plasmid # 74660 ; http://n2t.net/addgene:74660 ; RRID:Addgene_74660)