pET28a-Hook3 aa 1-552
(Plasmid #74614)

• Purpose: Expresses a truncation of Hook3 in bacteria for purification
• Depositing Lab: Ron Vale
• Publication: Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. doi: 10.1083/jcb.201604002.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 6198
• Total vector size (bp): 7854
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3) for protein production
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human Hook3 amino acids 1-552
• Alt name: HOOK3
• Species: H. sapiens (human)
• Insert Size (bp): 1656
• GenBank ID: NM_032410
• Entrez Gene: HOOK3 (a.k.a. HK3)
• Tags / Fusion Proteins:
  • superfolder GFP (N terminal on insert)
  • Strep II (N terminal on insert)
  • 6x His (N terminal on insert)

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry