pMSCVBEX1-WT-Myc-FLAG
(Plasmid
#74506)
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PurposeRetroviral expression of BEX1
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBEX1
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SpeciesH. sapiens (human)
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Entrez GeneBEX1 (a.k.a. BEX2, HBEX2, HGR74-h)
- Promoter unknown
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Tags
/ Fusion Proteins
- Myc (C terminal on backbone)
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer MSCV_C_rev2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVBEX1-WT-Myc-FLAG was a gift from Lars Rönnstrand (Addgene plasmid # 74506 ; http://n2t.net/addgene:74506 ; RRID:Addgene_74506) -
For your References section:
BEX1 acts as a tumor suppressor in acute myeloid leukemia. Lindblad O, Li T, Su X, Sun J, Kabir NN, Levander F, Zhao H, Lu G, Ronnstrand L, Kazi JU. Oncotarget. 2015 Aug 28;6(25):21395-405. 10.18632/oncotarget.4095 PubMed 26046670