G1S-HA-pCB6
(Plasmid
#74373)
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PurposeMammalian expression of a G1S mutant HA from the X:31 strain of influenza virus
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCB6
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHA
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MutationG1S
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G1S-HA-pCB6 was a gift from Judith White (Addgene plasmid # 74373 ; http://n2t.net/addgene:74373 ; RRID:Addgene_74373) -
For your References section:
A specific point mutant at position 1 of the influenza hemagglutinin fusion peptide displays a hemifusion phenotype. Qiao H, Armstrong RT, Melikyan GB, Cohen FS, White JM. Mol Biol Cell. 1999 Aug;10(8):2759-69. 10.1091/mbc.10.8.2759 PubMed 10436026