pISH-Emx2
(Plasmid #74346)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: empty spiracles homeobox 2
• Alt name: Emx2
• Species: M. musculus (mouse)
• Insert Size (bp): 668
• GenBank ID: NM_010132.2
• Entrez Gene: Emx2 (a.k.a. Pdo)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SpeI digestion and T7 RNA polymerase for antisense probe