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PurposeA lentiviral vector for the expression of mouse MYRF under doxycyline control.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 64843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-Tight-Puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7791
- Total vector size (bp) 11115
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-Tight-Puro_MYRF was a gift from Paul Tesar (Addgene plasmid # 64843 ; http://n2t.net/addgene:64843 ; RRID:Addgene_64843) -
For your References section:
Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, Karl RT, Maeda T, Miller RH, Tesar PJ. Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 2013 Apr 14. 10.1038/nbt.2561 PubMed 23584611