pISH-Dlx3
(Plasmid #74342)

• Purpose: Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.
• Depositing Lab: Peter Mombaerts
• Publication: Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: distal-less homeobox 3
• Alt name: Dlx3
• Species: M. musculus (mouse)
• Insert Size (bp): 829
• GenBank ID: NM_010055.3
• Entrez Gene: Dlx3 (a.k.a. Dlx-3)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NcoI digestion and SP6 RNA polymerase for antisense probe