pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
(Plasmid #74102)

• Purpose: plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
• Depositing Lab: Ben Szaro
• Publication: Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20.

Backbone

• Vector backbone: pSPORT1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4110
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Xenopus laevis beta-tubulin gene promoter region and 5'UTR
• Species: X. laevis (frog)
• Insert Size (bp): 3800
• GenBank ID: EF989124

Gene/Insert 2

• Gene/Insert name: Green Lantern Green Fluorescent Protein
• Insert Size (bp): 730

Gene/Insert 3

• Gene/Insert name: Rabbit beta 1-globin gene 3'UTR
• Species: O. cuniculus
• Insert Size (bp): 2184

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Purchased as pGreenLantern from GIBCO BRL

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry