pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
(Plasmid #74009)

• Purpose: Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter
• Depositing Lab: Filippo Del Bene
• Publication: Di Donato et al Genome Res. 2016 Mar 8.

Backbone

• Vector backbone: pminiTol2
• Backbone size w/o insert (bp): 3459
• Total vector size (bp): 9668
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: 5x UAS
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 382

Gene/Insert 2

• Gene/Insert name: Cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4150

Gene/Insert 3

• Gene/Insert name: T2A
• Insert Size (bp): 63

Gene/Insert 4

• Gene/Insert name: GFP
• Insert Size (bp): 714

Gene/Insert 5

• Gene/Insert name: U6 promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 331

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry