FU- dio PSD95-mCherry- W
(Plasmid #73919)

• Purpose: Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.
• Depositing Lab: Elly Nedivi
• Publication: Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010.

Backbone

• Vector backbone: FUW
• Backbone size w/o insert (bp): 9500
• Total vector size (bp): 12437
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PSD-95-mCherry
• Species: R. norvegicus (rat)
• GenBank ID: 13385
• Promoter: Ubi-C
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: Age1 (not destroyed)
• 5′ sequencing primer: GCTAGCCGCCACCATGGAC
• 3′ sequencing primer: ttacttgtacagctcgtcc

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).