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Addgene

FU - dio YFP - W
(Plasmid #73858)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73858 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFUGW
  • Backbone size w/o insert (bp) 9421
  • Total vector size (bp) 10138
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eYFP
  • Alt name
    enhanced yellow fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    717

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this backbone is prone to recombination. Addgene recommends that, upon receipt of this plasmid, users please streak onto an LB plate and screen single colonies by restriction digest before subsequent growth, storage, and experimentation.

This plasmid can be used with the low-efficiency Cre in Plasmid 101242: pSin wPGK-Cre ( https://www.addgene.org/101242/ ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FU - dio YFP - W was a gift from Elly Nedivi (Addgene plasmid # 73858 ; http://n2t.net/addgene:73858 ; RRID:Addgene_73858)
  • For your References section:

    Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E. Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. 10.1016/j.neuron.2016.01.010 PubMed 26853302