lenti sgRNA(MS2)_puro optimized backbone Citations (14)
Originally described in: Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening.Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed Journal
Articles Citing lenti sgRNA(MS2)_puro optimized backbone
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Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening.
Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F.
Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23.
PubMed
Associated Plasmids |
| Activation of the NRF2 antioxidant program generates an imbalance in central carbon metabolism in cancer. Sayin VI, LeBoeuf SE, Singh SX, Davidson SM, Biancur D, Guzelhan BS, Alvarez SW, Wu WL, Karakousi TR, Zavitsanou AM, Ubriaco J, Muir A, Karagiannis D, Morris PJ, Thomas CJ, Possemato R, Vander Heiden MG, Papagiannakopoulos T. Elife. 2017 Oct 2;6. doi: 10.7554/eLife.28083. PubMed |
| Reversible Regulation of Polyubiquitin Gene UBC via Modified Inducible CRISPR/Cas9 System. Han SW, Jung BK, Park SH, Ryu KY. Int J Mol Sci. 2019 Jun 28;20(13). pii: ijms20133168. doi: 10.3390/ijms20133168. PubMed |
| A conserved miRNA-183 cluster regulates the innate antiviral response. Singaravelu R, Ahmed N, Quan C, Srinivasan P, Ablenas CJ, Roy DG, Pezacki JP. J Biol Chem. 2019 Dec 20;294(51):19785-19794. doi: 10.1074/jbc.RA119.010858. Epub 2019 Nov 6. PubMed |
| Artificial escape from XCI by DNA methylation editing of the CDKL5 gene. Halmai JANM, Deng P, Gonzalez CE, Coggins NB, Cameron D, Carter JL, Buchanan FKB, Waldo JJ, Lock SR, Anderson JD, O'Geen H, Segal DJ, Nolta J, Fink KD. Nucleic Acids Res. 2020 Mar 18;48(5):2372-2387. doi: 10.1093/nar/gkz1214. PubMed |
| An MXD1-derived repressor peptide identifies noncoding mediators of MYC-driven cell proliferation. Raffeiner P, Hart JR, Garcia-Caballero D, Bar-Peled L, Weinberg MS, Vogt PK. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6571-6579. doi: 10.1073/pnas.1921786117. Epub 2020 Mar 10. PubMed |
| CRISPR activation of endogenous genes reprograms fibroblasts into cardiovascular progenitor cells for myocardial infarction therapy. Jiang L, Liang J, Huang W, Ma J, Park KH, Wu Z, Chen P, Zhu H, Ma JJ, Cai W, Paul C, Niu L, Fan GC, Wang HS, Kanisicak O, Xu M, Wang Y. Mol Ther. 2022 Jan 5;30(1):54-74. doi: 10.1016/j.ymthe.2021.10.015. Epub 2021 Oct 20. PubMed |
| Identification of Drug Transporter Genomic Variants and Inhibitors That Protect Against Doxorubicin-Induced Cardiotoxicity. Magdy T, Jouni M, Kuo HH, Weddle CJ, Lyra-Leite D, Fonoudi H, Romero-Tejeda M, Gharib M, Javed H, Fajardo G, Ross CJD, Carleton BC, Bernstein D, Burridge PW. Circulation. 2022 Jan 25;145(4):279-294. doi: 10.1161/CIRCULATIONAHA.121.055801. Epub 2021 Dec 7. PubMed |
| CRISPR activation screen identifies TGFbeta-associated PEG10 as a crucial tumor suppressor in Ewing sarcoma. Saratov V, Ngo QA, Pedot G, Sidorov S, Wachtel M, Niggli FK, Schafer BW. Sci Rep. 2022 Jun 23;12(1):10671. doi: 10.1038/s41598-022-12659-7. PubMed |
| Single-nucleus multi-omics of human stem cell-derived islets identifies deficiencies in lineage specification. Augsornworawat P, Hogrebe NJ, Ishahak M, Schmidt MD, Marquez E, Maestas MM, Veronese-Paniagua DA, Gale SE, Miller JR, Velazco-Cruz L, Millman JR. Nat Cell Biol. 2023 Jun;25(6):904-916. doi: 10.1038/s41556-023-01150-8. Epub 2023 May 15. PubMed |
| Integrative CRISPR Activation and Small Molecule Inhibitor Screening for lncRNA Mediating BRAF Inhibitor Resistance in Melanoma. Shamloo S, Kloetgen A, Petroulia S, Hockemeyer K, Sievers S, Tsirigos A, Aifantis I, Imig J. Biomedicines. 2023 Jul 21;11(7):2054. doi: 10.3390/biomedicines11072054. PubMed |
| Targeted CRISPR activation and knockout screenings identify novel doxorubicin transporters. Li Y, Tan M, Sun S, Stea E, Pang B. Cell Oncol (Dordr). 2023 Jul 31. doi: 10.1007/s13402-023-00847-0. PubMed |
| MAP3K1 regulates female reproductive tract development. Kimura E, Mongan M, Xiao B, Christianto A, Wang J, Carreira VS, Bolon B, Zhang X, Burns KA, Biesiada J, Medvedovic M, Puga A, Xia Y. Dis Model Mech. 2024 Mar 1;17(3):dmm050669. doi: 10.1242/dmm.050669. Epub 2024 Mar 28. PubMed |
| Generation of human cerebral organoids with a structured outer subventricular zone. Walsh RM, Luongo R, Giacomelli E, Ciceri G, Rittenhouse C, Verrillo A, Galimberti M, Bocchi VD, Wu Y, Xu N, Mosole S, Muller J, Vezzoli E, Jungverdorben J, Zhou T, Barker RA, Cattaneo E, Studer L, Baggiolini A. Cell Rep. 2024 Apr 23;43(4):114031. doi: 10.1016/j.celrep.2024.114031. Epub 2024 Apr 6. PubMed |
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