pSIREN-RetroQ-shLuc
(Plasmid
#73666)
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PurposepSRIEN-RetroQ vector containing control sequence luciferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepSIREN RetroQ
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Backbone manufacturerClonetech
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshRNA to Luciferase
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gRNA/shRNA sequenceLuciferase
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer U6 Promoter (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIREN-RetroQ-shLuc was a gift from Joe Landry (Addgene plasmid # 73666 ; http://n2t.net/addgene:73666 ; RRID:Addgene_73666) -
For your References section:
BPTF inhibits NK cell activity and the abundance of natural cytotoxicity receptor co-ligands. Mayes K, Elsayed Z, Alhazmi A, Waters M, Alkhatib SG, Roberts M, Song C, Peterson K, Chan V, Ailaney N, Malapati P, Blevins T, Lisnic B, Dumur CI, Landry JW. Oncotarget. 2017 May 12;8(38):64344-64357. doi: 10.18632/oncotarget.17834. eCollection 2017 Sep 8. 10.18632/oncotarget.17834 PubMed 28969075