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Addgene

pCDNA3-HA-Akt1
(Plasmid #73408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AKT1
  • Species
    H. sapiens (human)
  • Entrez Gene
    AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-HA-Akt1 was a gift from Jie Chen (Addgene plasmid # 73408 ; http://n2t.net/addgene:73408 ; RRID:Addgene_73408)

  • For your References section:

    Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Zeng M, van der Donk WA, Chen J. Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 10.1091/mbc.E14-01-0004. Epub 2014 Oct 1. 10.1091/mbc.E14-01-0004 PubMed 25273559
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