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Addgene

pJK276
(Plasmid #73366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2
  • Backbone size w/o insert (bp) 5857
  • Total vector size (bp) 7111
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    TOP10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    plastid omega-3 fatty acid desaturase
  • Alt name
    FAD7
  • Species
    Crepis alpina
  • Mutation
    Mutation I231F. Also insert is missing residues 1-33 of the 59 amino acid plastidal targeting sequence. No in-frame initiator ATG codon is present in the remaining transit peptide.
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer pYESTrp_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert contains an internal BamHI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK276 was a gift from T. J. Kappock (Addgene plasmid # 73366 ; http://n2t.net/addgene:73366 ; RRID:Addgene_73366)
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