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Addgene

pLVX-FLAG-mCherry-C1-centrin-1
(Plasmid #73333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-FLAG-mCherry-C1
  • Backbone manufacturer
    Modified Clontech
  • Backbone size w/o insert (bp) 8700
  • Total vector size (bp) 9245
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    centrin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    519
  • Entrez Gene
    CETN1 (a.k.a. CEN1, CETN)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG and mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer CMV seq
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-FLAG-mCherry-C1-centrin-1 was a gift from Manuel Thery (Addgene plasmid # 73333 ; http://n2t.net/addgene:73333 ; RRID:Addgene_73333)
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