pLVX-EGFP-C1-centrin-1
(Plasmid #73331)

• Purpose: Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
• Depositing Lab: Manuel Thery
• Publication: pLVX-EGFP-C3-Cep123 (unpublished)

Backbone

• Vector backbone: pLVX-EGFP-C1
• Backbone manufacturer: Modified Clontech
• Backbone size w/o insert (bp): 8800
• Total vector size (bp): 9305
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: centrin-1
• Species: H. sapiens (human)
• Insert Size (bp): 519
• Entrez Gene: CETN1 (a.k.a. CEN1, CETN)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: EGFP 3' Fw

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry