Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
(Plasmid #73164)

• Purpose: multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells
• Depositing Lab: Bruce Edgar
• Publication: Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi: 10.1016/j.celrep.2014.03.020. Epub 2014 Apr 13.

Backbone

• Vector backbone: Ac5-STABLE2-neo
• Backbone manufacturer: (Plasmid #32426)
• Vector type: Insect Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR
• Species: D. melanogaster (fly)
• Insert Size (bp): 3924
• Mutation: Cyclin B Fragment aa 1-266 & E2F1 Fragment aa 1-230
• Entrez Gene: E2f1 (a.k.a. Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, Dmel\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729)
• Entrez Gene: CycB (a.k.a. Dmel_CG3510, 2g, 3510, CG3510, CYC-B, CYCB, CYCLIN B, Cyc B, Cycb, Cyclin B1, DmcycB, Dmel\CG3510, chr2R:18312808..18312907, cyc B, cyc-B, cycB, cycb, cyclin B, cyclinB, cyclinB1, mAbF2F4)
• Tag / Fusion Protein:
  • mRFP1, GFP (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AC5_PRIMER
• 3′ sequencing primer: EBVrev

Resource Information

• Supplemental Documents:
  • GeneBank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry